manual de maquinas herramientas kibbe pdf

Isolation of bacteria from diabetic foot ulcers with special reference to anaerobe isolation by simple two-step combustion technique in candle jarĭirectory of Open Access Journals (Sweden) Evidently, the Anoxomat method is more reliable and appears to support the growth of strict anaerobes better. The Anoxomat system provided superior growth, in terms of density and colony size, and achieved anaerobiosis more rapidly. The zones of inhibition of the antibiotics tested were not different in the two systems. The density of growth recorded for 177 (78%) strains was heavier in the Anoxomat than in the GasPak jar. With the Anoxomat, anaerobiosis was achieved about 35 min faster than in the GasPak system. The Porphyromonas asaccharolytica strains failed to grow within the GasPak system but grew inside the Anoxomat.

There was no measurable difference in the colony sizes of the reference strains. The remaining 12% were equal in their sizes.

Of the 227 strains tested, the colonies of 152 (67%) were larger (by size range of 0.2-2.4 mm) in the Anoxomat at 48 h than in the GasPak jar compared with only 21% (range 0.1-0.3 mm) that were larger in the GasPak than in the Anoxomat. The results demonstrate that the growth of anaerobic bacteria is faster inside the Anoxomat jar than in the anaerobic GasPak jar system. A total of 227 obligate anaerobic bacteria comprising 116 stock strains, 5 ATCC reference strains and 106 fresh strains, representing different genera, were investigated for growth on anaerobic agar plates and scored for density, colony sizes, susceptibility zones of antibiotic inhibition and the speed of anaerobiosis (reducing the indicator). An anaerobic indicator within the jars monitored anaerobiosis.

Anoxomat system utilized a gas mixture of 80% N(2), 10% CO(2) and 10% H(2), while the GasPak used a gas mixture of 90% H(2) and 10% CO(2). Anoxomat, model WS800, and anaerobic GasPak jar system (Oxoid) were evaluated. To evaluate the performance of the Anoxomat, in comparison with the conventional anaerobic GasPak jar system, for the isolation of obligate anaerobes. Shahin, May Jamal, Wafaa Verghese, Tina Rotimi, V O Comparative evaluation of anoxomat and conventional anaerobic GasPak jar systems for the isolation of anaerobic bacteria.

Manual de maquinas herramientas kibbe pdf